Allesgen Alternative Treatment
Three Phases of Experiments
Phase I: Tumor cell lines experiment
6 cancer cell lines were used for this research. Cancer cell lines from breast, lung, colon, ovarian, cervical and uterine origins were cultured and Allesgen in an amount of 0.2, 0.4, 0.6, 0.8, 1.0mg/ml concentration were administered in cell cultural solutions. The result are outstanding: 50% of all cancer cell lines were eliminated in the concentration of Allesgen in amount of 0.4 to 0.6 mg/ml, and more than 90% of cancer cell lines were eliminated at the concentration of 1mg/ml.
Phase II: Animal Experiment
Allesgen inhibits cancer cell growth in vivo on experiemntal animal.
Allesgen intraperitoneal administration to experimental animal.
 14 experimental animals of 4 to 6 week old white rabbits each weighting 1 to 1½ pounds were fed under a condition of 23+_3 degree C, relative humidity of 45+_5 % and photoperiod of 12 light/12 dark. The rabbits were divided into seven groups of 2 heads each and were fed with Harlan-Taklad rabbit diet TD-1376 containing moisture 12%, crude protein 16%, crude fat 2%, crude fiber 15% , ash 8%, and 47% of nitrogen free substances.
 The rabbits were fed for 3 weeks with free access to the diet and water. Body weight was recorded every 7 days, and records were analyzed. All rabbits showed a normal growth rate with no significant differences among the 7 groups in regard to the diet ingestion amount or the body weight gain. The cancer cell lines were injected into six groups of the rabbits, that is 2 heads each group with 2 heads serving as controls (without tumor cell injection). The cancer cell lines were developed from Example-2. Each head was injected 0.5ml of different cell line fluid intraperitoneally, prefer in the peritoneum layer, then the rabbits were fed the same diet for 3-4 weeks until a tumor grew in the peritoneum. The size and location of the tumors were recorded. When the tumors reached 3-5mm diameter in size, Allesgen in the amount of 25.0mg/ml in normal saline with 100mg of vitamin C (to keep the solution acidified), one ml of Allesgen was injected into the six different group of rabbits, the Allesgen were given twice a week for 8 weeks.
 After 8 weeks of treatment, the rabbits were anesthetized with injections of ketamine 75mg/kg in the femoral muscle and sacrificed. Blood samples were collected from the heart of each rabbit to determine the blood analysis which consisted of: complete blood count (CBC), Chemistry-7 and 24, (including liver and renal function tests), lipid profiles (including total cholesterol, HDL, LDL, VLDL, and triglycerides), coagulation factors consisting of; prothrombintime (PT), partial thromboplastin time (PPT), and immune-globulin –E. All the laboratory tests were analyzed, and indicated no differences among or within each groups. All laboratory blood analyses were performed on rabbits of all 7 groups. The results were tested using student t-test and Microsoft Excel-7 programs. The results are depicted in Table-II.
 Table–II, The table presents blood analysis of the rabbits of 6 different groups which were treated with Allesgen after inoculation of cancer cell lines. Control group; TC (183.3+_50.2mg/dl ), TRG (110+_40.6mg/dl), HDL (45.6+_20.4mg/dl), SGOT (38.6+_6.2u/l), SGPT (62.5 +_6.5u/l), GGTP (8+_ 2.4u/l ), WBC (6.8 +_ 2.0k/ul), Hb; (12.3+_2.2gm/dl). Bromelainase treated groups; TC (175.6+_ 36.8mg/dl), TRG (92.6 +_ 38.8mg/dl), HDL (43.6 +_16.5mg/dl), SGOT (110.8 +_30.7u/l ), SGPT (71.2 +_3.8u/l ), GGTP (7+_1), WBC (7.3+_ 2.2k/ul), Hb (11.9+_1.9gm/dl). TC; Total Cholesterol, TRG: Triglycerides, WBC: White Blood Cell, HDL; High Density Lipoprotein, SGOT; Serum Glutamo-Oxalic Transferase, SGPT; Serum Glutamo-PyruvicTransferase, Hb; Hemoglobin.
 The internal organs from the rabbits sacrified in this sample including lung, heart, liver, kidney, muscle, omentum, intestine, stomach bladder and pancreas were visual examined and showed no abnormalities. One half of each organ was frozen, and the other half was fixed with 10% neutral buffered formalin for 24 hours. Then the fixed organs were washed with tap waterand stepwise dehydrated with 70%, 80%, 90% and 100% ethanol and then embedded in paraffin using Shandon-Histocentre-2. The embedded organ blocks were sectioned in 4mm thickness with a microtome (McBain, M 820, American Optical Co. USA) and stained with hematoxylin and eosin stain (H.E. stain). The stained specimens were made transparent with xylene, and mounted with permount on microslides. There were no pathological abnormalities or lesions under microscopic examination. All the specimens collected from six group of rabbits showed no evidence of persistent disease or cancer cells.
Therefore, we concluded that Allesgen can served as a potent chemotherapeutic agent in various types of cancer treatment in experimental animal without side effects.